Research on the Role of Ezrin Gene in the Pathological Process of Cervical Cancer

Duan H, Zhang Z, Shi J, Chen Y and Li X

Department of Reproduction Gynecology, Northwest Women and Children’s Hospital, China
Department of Ultrasonography, The Affiliated Children Hospital of Xi’an, Jiaotong University Shaanxi, China
Reproductive Center, Northwest Women and Children’s Hospital, China
Department of Reproductive Medical Center, Xi’an No. 4 Hospital, China
These authors contributed equally to this work

^*Correspondance to: Xiaojuan Li 

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Abstract:

Aim: This paper is going to study the effect of Ezrin gene on the proliferation and migration of cervical cancer cells and to explore the key role of Ezrin gene in the pathogenesis of cervical cancer.
Method: RT-PCR and Western blot were used to detect the cervical cancer cell lines, HeLa and C33A, and the expression of Ezrin in cervical cancer tissue. After low-expression of Ezrin gene through RNA interfering technology, this paper observed the different expression of E-cadherin, MMP-2 and β-Catenin.
Result: The result from RT-PCR and Western blot showed that the expression of Ezrin protein was increasing in cervical cancer cell lines, HeLa, C33A and cervical cancer tissues, but not expressed in normal cervical tissues. After using the interfering RNA technology to express the Erzrin gene in the cervical cancer cell line, HeLa, the proliferative ability of HeLa cells was lower than that of the noninterference group. Meanwhile, the expression of E-cadherin protein was up-regulated after the low expression of Ezrin gene and the expression of MMP-2 and β-Catenin are increasing.
Conclusion: As a kind of cancer-promoting genes, Ezrin gene can activate EMT so as to induce proliferation, metastasis and infiltration of cervical cancer cells.

Keywords:

Ezrin; Cervical cancer; EMT

Cite the Article:

Duan H, Zhang Z, Shi J, Chen Y, Li X. Research on the Role of Ezrin Gene in the Pathological Process of Cervical Cancer. Clin Oncol. 2022;7:1968..