The Protective Effects of Hepatopoietin Cn (HPPCn) on Acute Liver Injury

Na Li, Feng-Jiao Liu, Chun-Xia Sun, Dan-Dan Li, Jian Li, Mei-Hua Qu, Chun-Ping Cui and Dajin Zhang

Department of Pharmacy, Weifang Medical University, China
Department of Medical Sciences, Sixth Medical Center of PLA 301 Hospital, China
Department of Protein Sciences, National Center of Protein Sciences (Beijing), China
Both Authors contributed equally

^*Correspondance to: Da-Jin Zhang 

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Abstract:

Objective: To observe the protective role of HPPC non acute liver injured.Methods: Six hours after 10 mmo1/L CC14, 150 mmol/L ethanol or 0.6 mmol/L H2O2 treatment, human hepatoma SMMC7721 cells were incubated with 10, 100, 200 ng/ml recombinant human HPPCn protein (rhHPPCn) for another 24 hrs. The cell survival rate was analyzed by CCK-8 assay. The CC14 induced apoptosis of SMMC7721 cells was detected by flow cytometry. Then the levels of GOT, GPT, MDA, LDH, GSH-PX and SOD in SMMC7721 cells lysates or cell culture supernatant were detected. SMMC7721 cells were treated with different concentrations of rhHPPCn (0, 10, 100 ng/ml). The expression of Cell proliferation indexes (Brdu and PCNA) were detected by Immuno Histo Chemistry (IHC). Acute liver injury mouse model was established by a one-time tail vein injection of 20% CCl4 at a volume of 5 ml/kg body weight. At one hour after the injection, 2.5 mg rhHPPCn/12h/kg body weights were administered through tail vein injection. The serum levels of GOT and GPT were detected and pathological changes in liver were evaluated. The expression changes of PCNA were observed by IHC.Results: rhHPPCn could increase the survival rate of SMMC7721 cells and inhibit chemical toxics-induced cell apoptosis. The cell supernatants levels of GOT, GPT, MDA, LDH were significantly reduced, while GSH-PX and SOD levels significantly increased after rhHPPCs treatment in the CCl4 treated SMMC7721 cells. The expression of Brdu and PCNA was increased by concentration dependent, and this effect, which indicates that rhHPPCn, can promote cell proliferation. In addition, rhHPPCn has a liver protective effect against liver injuries in vivo. It significantly reduced serum GOT, GPT levels in CCl4 induced acute liver injury mouse in a time and concentration-depended manner, and increased the expression of PCNA in liver significantly.Conclusion: rhHPPCn protects the hepatocytes from chemical agents by promoting proliferation and inhibiting apoptosis both in vivo and in vitro. Our study can provide new insights for clinical treatment of acute liver injury.

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Cite the Article:

Li N, Liu F-J, Sun C-X, Li D-D, Li J, Qu M-H, et al. The Protective Effects of Hepatopoietin Cn (HPPCn) on Acute Liver Injury. Clin Oncol. 2019; 4: 1587.