Flow Cytometry Analysis of DNA Ploidy and S-Phase Fraction in Salivary Gland Tumors of Egyptian Patients

Asmaa M Zahran, Hussein Fakhry, Khaled A Hussein, Mahmoud Abd El-Salam, Mohamed A Mohamed, Safaa M Tohamy and Ahmed M Hussein

1Department of Clinical Pathology, Assiut University, Egypt
Department of Surgical Oncology, Assiut University, Egypt
Department of Oral and Dental Pathology, Al-Azhar University, Egypt
Department of Oral Pathology, Minia University, Egypt
Department of Oral and Maxillofacial Pathology, South Valley University, Egypt

^*Correspondance to: Hussein Fakhry 

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Abstract:

Aim: The aim of this study is to investigate the DNA ploidy and S-Phase Fraction (SPF) of some Salivary Gland Tumors (SGTs) in Egyptian patients and to investigate the correlation between these two biological parameters and the presumptive behavior of these neoplasms.Methods: Flow cytometric analysis of DNA pliody and S-Phase Fraction (SPF) was done in 50 fresh tumor tissue sections of SGTs which diagnosed as 15 benign and 35 malignant tumors.Results: 93.3% of the benign SGTs tumors were diploid, while only 34% of malignant tumors were diploid and 66% were aneuploid. The malignant SGTs had higher SPF than the benign tumors but with no significant difference. There was no significant correlation of ploidy status or SPF with the tumor grades of mucoepidermoid carcinomas.Conclusion: DNA aneuploidy may be a key indicator for tumors activity and malignancy in SGTs, while the SPF has a minor role in the evaluation of SGTs activity.

Keywords:

Flow cytometry; Salivary gland neoplasm; Aneuploidy; Mitotic Index; Diploidy; Mucoepidermoid carcinoma

Cite the Article:

Zahran AM, Fakhry H, Hussein KA, Abd El-Salam M, Mohamed MA, Tohamy SM, et al. Flow Cytometry Analysis of DNA Ploidy and S-Phase Fraction in Salivary Gland Tumors of Egyptian Patients. Clin Oncol. 2018; 3: 1393.